منابع مشابه
Design Principles for Ligand-Sensing, Conformation-Switching Ribozymes
Nucleic acid sensor elements are proving increasingly useful in biotechnology and biomedical applications. A number of ligand-sensing, conformational-switching ribozymes (also known as allosteric ribozymes or aptazymes) have been generated by some combination of directed evolution or rational design. Such sensor elements typically fuse a molecular recognition domain (aptamer) with a catalytic s...
متن کاملRibozymes
The discovery in 1982 that an intron of the protozoan Tetrahymena can excise itself from precursor ribosomal RNA in the absence of protein challenged the belief that only proteins can act as biological catalysts. The new field of RNA enzymology emerged to study such phenomena, and catalytic RNAs have now been shown to fold up to form complex three-dimensional surfaces that can bind specific sub...
متن کاملIn vitro selection of adenine-dependent hairpin ribozymes.
Adenine-dependent hairpin ribozymes were isolated by in vitro selection from a degenerated hairpin ribozyme population. Two new adenine-dependent ribozymes catalyze their own reversible cleavage in the presence of free adenine. Both aptamers have Mg(2+) requirements for adenine-assisted cleavage similar to the wild-type hairpin ribozyme. Cleavage kinetics studies in the presence of various othe...
متن کاملLigand-dependent receptor down-regulation
The kinetics of internalization, sequestration and metabolic degradation of atrial natriuretic factor (ANF)-receptor complex were studied in rat thoracic aortic smooth-muscle (RTASM) cells. These parameters were directly determined by measuring 125I-ANF binding to total, intracellular and cell-surface receptors. Pretreatment of cells with the lysosomotropic agent chloroquine and the energy depl...
متن کاملSmall self-cleaving ribozymes.
The hammerhead, hairpin, hepatitis delta virus (HDV), Varkud Satellite (VS), and glmS ribozymes catalyze sequence-specific intramolecular cleavage of RNA. They range between 50 and 150 nucleotides in length, and are known as the "small self-cleaving ribozymes." Except for the glmS ribozyme that functions as a riboswitch in Gram-positive bacteria, they were originally discovered as domains of sa...
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ژورنال
عنوان ژورنال: Wiley Interdisciplinary Reviews: RNA
سال: 2016
ISSN: 1757-7004
DOI: 10.1002/wrna.1395